Endo-?-galactosidase from Bacteroides fragilis recombinant, expressed in E. coli, ?140 units/mg protein, buffered aqueous solution | Keratanase | Sigma-Aldrich. G6920 Sigma-Aldrich.
Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may decrease or eliminate cleavage. Endo-Beta-Galactosidase is useful for identifying and removing poly-N-acetyllactosamine structures on many biologically important glycoconjugates.
Search results for endo-?-galactosidase at Sigma-Aldrich, Digestion of glycolipids by endo- b -galactosidase 3)–5) 1) Glycosphingolipid (100 ?g) dissolved in 50 ?L 0.2 M sodium-acetate buffer, pH 5.8, containing 200 ?g sodium deoxy taurocholate. Comment 0: 2) Dissolve endo-?-galactosidase (0.1 Unit) with 100 ?L water, and.
References: 1. Scudder,P.
Uemura, K.
Doby, J.
Fukuda, M.N. & Feizi, T.(1983) Isolation and characterization of an endo- b -galactosidase from Bacteroides fragilis …
1/1/1982 · This chapter presents the procedure for purification and assay of endo-?-galactosidase from Eseheriehia freundii. In enzyme isolation The organism fro…, Endo- b -Galactosidase 1.2. Relevant identified uses of the substance or mixture and uses advised against Laboratory chemicals For research use only. Not intended for human or animal diagnostic or therapeutic uses or used in the manufacture of food or pharmaceutical products 1.3. Details of the supplier of the safety data sheet, Endo- B -galactosidase thus purified showed about 20 times higher specific activity and improved recovery compared to the orig- inal method (Table 11; Fig. 2). The affinity chromatography greatly removed the contaminated protein which could not be eliminated by conventional method (see Fig. 2,.
12/1/1982 · DISCUSSION The endo-~-galactosidase from Escherichia freundii is an induced enzyme (3), whereas the endo-B-galactosidase of suitable strains of Bacteroides fragilis or Flavobacterium keratolyticus are produced naturally, and are therefore found in culture supernatants which can be used to prepare Tk-erythrocytes in vitro.
Porcine embryonic fibroblasts were transfected with pCEIEnd, an expression vector that simultaneously expresses enhanced green fluorescent protein (EGFP) and endo- b -galactosidase C(EndoGalC; an enzyme capable of digesting cell surface a-Gal epitope) upon transfection.
